is the study of the rates
of chemical reactions
that are catalysed
. The study of an enzyme's kinetics
provides insights into the catalytic mechanism of this enzyme, its role in metabolism
, how its activity is controlled in the cell
and how drugs
Enzymes are molecules that manipulate other molecules — the enzymes' substrates. These target molecules bind to an enzyme's active site and are transformed into products through a series of steps known as the enzymatic mechanism. Some enzymes bind multiple substrates and/or release multiple products, such as a protease cleaving one protein substrate into two polypeptide products. Others join substrates together, such as DNA polymerase linking a nucleotide to DNA. Although these mechanisms are often a complex series of steps, there is typically one rate-determining step that determines the overall kinetics. This rate-determining step may be a chemical reaction or a conformational change of the enzyme or substrates, such as those involved in the release of product(s) from the enzyme.
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